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rabbit polyclonal anti neun  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit polyclonal anti neun
    Rabbit Polyclonal Anti Neun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+neun/DJ-1+Antibody/bio_rxiv__2025__07__07__663457-288-23-27
    Average 93 stars, based on 123 article reviews
    rabbit polyclonal anti neun - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: Metabotropic glutamate receptor 5 inhibits α-synuclein-induced microglia inflammation to protect from neurotoxicity in Parkinson’s disease
    Article Snippet: After washing in PBS, the sections were incubated with Alexa Fluor 594 donkey anti-rabbit (1:200, Invitrogen), Alexa Fluor 647 donkey anti-goat (1:200, Invitrogen), Alexa Fluor 488 donkey anti-mouse (1:200, Invitrogen), Alexa Fluor 405 donkey anti-rat (1:100, Abcam), Alexa Fluor 488 donkey anti-rabbit (1:200, Invitrogen), and Alexa Fluor 594 donkey anti-mouse (1:200, Invitrogen) for 1 h in the dark. .. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Cell Signaling Technology) for 5 min. Primary antibodies included rabbit polyclonal anti-mGluR5 (1:200, EMD Millipore), goat monoclonal anti-Iba-1 (1:200; Abcam), mouse monoclonal anti-α-syn (1:100, BD Biosciences), rat monoclonal anti-lysosome-associated membrane protein (LAMP)-1 (1:50, Santa Cruz Biotechnology), rabbit polyclonal anti-NeuN (1:50; Cell Signaling Technology). and mouse monoclonal anti-TH (1:2000, Sigma-Aldrich). .. Images were captured using a confocal microscope (TCS SP8; Leica, Solms, Germany).

    Cell-Signaling:

    Article Title: A novel cholinergic projection from the lateral parabrachial nucleus and its role in methamphetamine-primed conditioned place preference
    Article Snippet: .. The following primary antibodies were used: goat polyclonal anti-ChAT (1:200, RRID: AB_2079751, Millipore, USA), rabbit polyclonal anti-NeuN (1:200, RRID: AB_2651140, Cell Signaling Technology, USA) and rabbit polyclonal anti-c-Fos (1:1500, RRID: AB_2247211, Cell Signaling Technology, USA). .. All virus samples in the present study were packaged by BrainVTA (Wuhan, China).

    Blocking Assay:

    Article Title: Astrocytic mGluR5 signaling tunes emotional and cognitive processing in the adult brain
    Article Snippet: Free-floating tissue was first washed in PBS and then permeabilized with a 0.3% Triton X-100 (Sigma Aldrich, USA) in PBS (0.3% PBS-T) solution for 10 min. To reduce nonspecific binding, the tissue was incubated with 10% normal goat serum (NGS, Abcam) in 0.3% PBS-T blocking solution for 1 h at room temperature (RT). .. This blocking step was followed by overnight incubation at 4 °C with the primary antibodies rabbit anti-GFAP (1:200, Abcam, UK), rabbit anti-mGluR5 (1:200), rabbit polyclonal anti-NeuN (1:100; Cell Signalling, USA), rabbit polyclonal anti-iba1 (1:750; FUJIFILM Wako Chemicals, Hong Kong), rabbit polyclonal anti-S100β (1:200; Abcam, UK), rabbit polyclonal anti CC-1 (1:100, Millipore, USA), mouse anti-GFAP (1:200, Abcam, UK), and chicken anti-mCherry (1:500; HenBiotech, Portugal), in 2% NGS and 0.3% PBS-T. On the next day, the slices were washed in 0.3% PBS-T and incubated with the respective species-specific secondary antibody: goat anti-rabbit Alexa Fluor® 488 (1:1000; Thermo Fischer Scientific, USA), goat anti-mouse Alexa Fluor® 594 (1:1000; Thermo Fischer Scientific, USA), goat anti-chicken Alexa Fluor®594 (1:1000; Invitrogen, TermoFisher Scientific, USA), or goat anti-mouse®647 (1:1000; Thermo Fischer Scientific, USA), in a 0.3% PBS-T solution with 2% NGS, for 1 h at RT. .. After this period, the slices were washed with 0.3% PBS-T, and the nucleic acids were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Invitrogen, USA) for 10 min at RT.

    Incubation:

    Article Title: Astrocytic mGluR5 signaling tunes emotional and cognitive processing in the adult brain
    Article Snippet: Free-floating tissue was first washed in PBS and then permeabilized with a 0.3% Triton X-100 (Sigma Aldrich, USA) in PBS (0.3% PBS-T) solution for 10 min. To reduce nonspecific binding, the tissue was incubated with 10% normal goat serum (NGS, Abcam) in 0.3% PBS-T blocking solution for 1 h at room temperature (RT). .. This blocking step was followed by overnight incubation at 4 °C with the primary antibodies rabbit anti-GFAP (1:200, Abcam, UK), rabbit anti-mGluR5 (1:200), rabbit polyclonal anti-NeuN (1:100; Cell Signalling, USA), rabbit polyclonal anti-iba1 (1:750; FUJIFILM Wako Chemicals, Hong Kong), rabbit polyclonal anti-S100β (1:200; Abcam, UK), rabbit polyclonal anti CC-1 (1:100, Millipore, USA), mouse anti-GFAP (1:200, Abcam, UK), and chicken anti-mCherry (1:500; HenBiotech, Portugal), in 2% NGS and 0.3% PBS-T. On the next day, the slices were washed in 0.3% PBS-T and incubated with the respective species-specific secondary antibody: goat anti-rabbit Alexa Fluor® 488 (1:1000; Thermo Fischer Scientific, USA), goat anti-mouse Alexa Fluor® 594 (1:1000; Thermo Fischer Scientific, USA), goat anti-chicken Alexa Fluor®594 (1:1000; Invitrogen, TermoFisher Scientific, USA), or goat anti-mouse®647 (1:1000; Thermo Fischer Scientific, USA), in a 0.3% PBS-T solution with 2% NGS, for 1 h at RT. .. After this period, the slices were washed with 0.3% PBS-T, and the nucleic acids were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Invitrogen, USA) for 10 min at RT.

    Article Title: Adenosine deaminase acting on RNA 2 provides neuroprotection by activating Kv1.1 channels in a rat epilepsy model
    Article Snippet: .. The sections were blocked with 3% (v/v) bovine serum albumin (BSA) and incubated with 100 mL diluted primary antibody of rabbit polyclonal anti-NeuN (24307, 1:1000, Cell Signaling Technology) overnight at 4°C and then incubated with horseradish peroxidase (HRP)-conjugated secondary antibody for 1 h at room temperature. .. The reaction was visualized through incubation of the sections with a Diaminobenzidine kit (ZLI-9017, ZSGB-BIO, Beijing, China).

    Article Title: Genetically modified mouse expressing human APOE4 and mouse Trem2 p.R47H and methods of use thereof
    Article Snippet: Cryosections were rinsed with PBT (1×PBS with 1% Triton-100) for 5 minutes (mins) then incubated with 500 μL of Liberate Antibody Binding Solution (L.A.B.-Polysciences Inc.) solution for 20 minutes at room temperature (RT) for antigen retrieval. .. Slides were then incubated overnight at 4° C. in the following primary antibodies: rabbit polyclonal anti-GFAP (1:200, Dako); rabbit polyclonal anti-IBA1 (1:250, Wako); rabbit polyclonal anti-NeuN (1:100, Cell Signaling Inc); mouse monoclonal anti-non-phosphorylated neurofilament (1:200, Covance) and sheep polyclonal anti-TREM2 (1:200, RD Systems). ..

    Next-Generation Sequencing:

    Article Title: Astrocytic mGluR5 signaling tunes emotional and cognitive processing in the adult brain
    Article Snippet: Free-floating tissue was first washed in PBS and then permeabilized with a 0.3% Triton X-100 (Sigma Aldrich, USA) in PBS (0.3% PBS-T) solution for 10 min. To reduce nonspecific binding, the tissue was incubated with 10% normal goat serum (NGS, Abcam) in 0.3% PBS-T blocking solution for 1 h at room temperature (RT). .. This blocking step was followed by overnight incubation at 4 °C with the primary antibodies rabbit anti-GFAP (1:200, Abcam, UK), rabbit anti-mGluR5 (1:200), rabbit polyclonal anti-NeuN (1:100; Cell Signalling, USA), rabbit polyclonal anti-iba1 (1:750; FUJIFILM Wako Chemicals, Hong Kong), rabbit polyclonal anti-S100β (1:200; Abcam, UK), rabbit polyclonal anti CC-1 (1:100, Millipore, USA), mouse anti-GFAP (1:200, Abcam, UK), and chicken anti-mCherry (1:500; HenBiotech, Portugal), in 2% NGS and 0.3% PBS-T. On the next day, the slices were washed in 0.3% PBS-T and incubated with the respective species-specific secondary antibody: goat anti-rabbit Alexa Fluor® 488 (1:1000; Thermo Fischer Scientific, USA), goat anti-mouse Alexa Fluor® 594 (1:1000; Thermo Fischer Scientific, USA), goat anti-chicken Alexa Fluor®594 (1:1000; Invitrogen, TermoFisher Scientific, USA), or goat anti-mouse®647 (1:1000; Thermo Fischer Scientific, USA), in a 0.3% PBS-T solution with 2% NGS, for 1 h at RT. .. After this period, the slices were washed with 0.3% PBS-T, and the nucleic acids were labeled with 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Invitrogen, USA) for 10 min at RT.

    Immunofluorescence:

    Article Title: Demonstration of therapeutic effect of plasma-synthesized polypyrrole/iodine biopolymer in rhesus monkey with complete spinal cord section.
    Article Snippet: .. Immunofluorescence assay was done in 8 mm sagittal sections of spinal cord by using specific primary antibodies for neurons: rabbit polyclonal anti NeuN (1:250; Cell Signaling Technology; Danvers, Massachusetts, USA) and mouse monoclonal anti βIII-tubulin (1:300; Abcam; Cambridge, UK). .. After incubation, the tissues were washed and incubated with suitable secondary antibodies: Alexa Fluor 594 donkey anti-mouse (1:500; Invitrogen Molecular Probes; Eugene, Oregon, USA) and Alexa Fluor 488 donkey anti-rabbit.



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    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), <t>NeuN</t> (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .
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    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), <t>NeuN</t> (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .
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    FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of <t>NeuN</t> expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.
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    Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers <t>NeuN,</t> TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.
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    Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers <t>NeuN,</t> TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.
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    Image Search Results


    Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .

    Journal: Research

    Article Title: Aerobic Exercise-Induced TGF-β Receptor Reprogramming Disrupts Neutrophil–Microglia Crosstalk to Attenuate Early Brain Injury after Subarachnoid Hemorrhage

    doi: 10.34133/research.1301

    Figure Lengend Snippet: Animal study verifying the effect of AE in mitigating EBI of SAH. (A) The complete timeline of an experimental plan, schematic diagram, and actual skull base image of the SAH/Sham model in this animal experiment. Some elements are created in BioRender (2026); https://BioRender.com/vwwir98 . (B) SAH grading score of each group in this stage. n = 24. (C) Modified Garcia score of each group, n = 24. (D) The water content of the left and right brains in different groups at this stage. n = 6. (E and F) Apoptosis of bleeding focus cortical neurons was detected by TUNEL assay (red), NeuN (green), and DAPI (blue) immunofluorescence. n = 6. Scale bars, 50 μm. (G) Bars show changes in BBs in SAH mice. n = 24. (H to N) Western blotting images and quantitative data of relative expression level of Il-1b, Il-6, Nos2, Il-4, Il-10, and Tgfb1 in the ipsilateral cortex after SAH. n = 6. (O) The coronal sections were stained with hematoxylin and eosin (H&E) for histological evaluation AE-regulated in SAH. n = 6. Scale bars, 50 μm. (P) Histopathological changes detected by Nissl’s staining in hippocampus and the hemorrhagic cortex. n = 6. Scale bars, 50 μm. In (B), (C), and (G), data were represented as median (interquartile range), and P values were calculated using Kruskal–Wallis H with Dunn test. In (D), (F), and (I) to (N), data were represented as mean ± SD, and P values were calculated using one-way ANOVA with Tukey multiple comparisons. * P < 0.05, ** P < 0.01, *** P < 0.001. The original Western blot images are shown in Data . Detailed experimental data and statistical results, including Z and P values for Kruskal–Wallis test and F and P values for ANOVAs, are provided in Data to .

    Article Snippet: After fixation, the tissue was processed for TUNEL assay with the cell apoptosis detection kit (Servicebio, G1502-50T), and Rabbit anti-NeuN polyclonal antibody (ABclonal, A0951) and its corresponding secondary antibody: Donkey anti-rabbit IgG conjugated to Alexa Fluor 488 (Antgene, ANT024, diluted 1:400).

    Techniques: Modification, TUNEL Assay, Immunofluorescence, Western Blot, Expressing, Staining

    FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of NeuN expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.

    Journal: Frontiers in Medicine

    Article Title: Gastrodia elata fermentation alleviates methamphetamine-induced neuroinflammation and anxiety- and depression-like behaviors by regulating the PI3K-AKT signaling pathway

    doi: 10.3389/fmed.2026.1768944

    Figure Lengend Snippet: FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of NeuN expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.

    Article Snippet: SP Rabbit/Mouse HRP Kit (DAB) (Cat# SA00001-2/SA00001-1, CWBIO, China); DAB Substrate Kit (Cat# ZLI-9018, Zhongshan Jinqiao, Beijing, China); SPARKscript II RT Kit/SYBR Green RT-PCR Kit (Cat# AG0305-B/AH0104-B, SparkJade, China); Anti-Iba1 Rabbit Polyclonal Antibody (Cat# F155306, Abways, China); Anti-GFAP Rabbit Polyclonal Antibody (Cat# CY5424, Abcam, United Kingdom); Anti-NeuN Rabbit Polyclonal Antibody (Cat# 26975-1-AP, Proteintech, China); AKT/phosphorylated AKT (p-AKT)/phosphorylated PI3K (p-PI3K)/GAPDH (Cat# 10176-2-AP/66444-1-IG/20584-1-AP/60004-1-IG, Proteintech, China); PI3K (Cat# AF3242, Affinity, China).

    Techniques: Immunohistochemical staining, Expressing

    Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers NeuN, TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.

    Journal: Experimental Biology and Medicine

    Article Title: Curcumin-enhanced elvitegravir therapy mitigates neuroinflammation and cognitive deficits in EcoHIV mice

    doi: 10.3389/ebm.2025.10758

    Figure Lengend Snippet: Brain levels of 8-OHdG (A,B) and glutamate (C,D) were measured in EcoHIV-infected mice treated with EVG, CUR, or their combination (EVG + CUR) via IN or IP routes. (E–I) Western blot analysis was conducted to assess the expression of neural protein markers NeuN, TMEM119, synaptophysin, L1CAM, and GFAP in the mice brains. Data are expressed as mean ± SEM (n = 4 per group). Statistical analysis was performed by one-way ANOVA with Tukey’s post hoc test. *, **, and *** represents p ≤ 0.05, p ≤ 0.01, and p ≤ 0.001 respectively.

    Article Snippet: Primary antibody incubation was carried out overnight at 4 °C using the following antibodies: NeuN rabbit polyclonal antibody (1:1000, Proteintech, Cat# 26975-1-AP), synaptophysin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 67864-1-Ig), GFAP rabbit polyclonal antibody (1:1000), L1CAM rabbit polyclonal antibody (Proteintech, Cat# 20659-1-AP), and β-actin mouse monoclonal antibody (1:20,000, Proteintech, Cat# 66009-1-Ig) as an internal loading control.

    Techniques: Infection, Western Blot, Expressing