rabbit polyclonal anti neun (Cell Signaling Technology Inc)
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Rabbit Polyclonal Anti Neun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+neun/DJ-1+Antibody/bio_rxiv__2025__07__07__663457-288-23-27
Average 93 stars, based on 123 article reviews
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Membrane:Article Title: Metabotropic glutamate receptor 5 inhibits α-synuclein-induced microglia inflammation to protect from neurotoxicity in Parkinson’s disease Article Snippet: After washing in PBS, the sections were incubated with Alexa Fluor 594 donkey anti-rabbit (1:200, Invitrogen), Alexa Fluor 647 donkey anti-goat (1:200, Invitrogen), Alexa Fluor 488 donkey anti-mouse (1:200, Invitrogen), Alexa Fluor 405 donkey anti-rat (1:100, Abcam), Alexa Fluor 488 donkey anti-rabbit (1:200, Invitrogen), and Alexa Fluor 594 donkey anti-mouse (1:200, Invitrogen) for 1 h in the dark. .. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Cell Signaling Technology) for 5 min. Primary antibodies included rabbit polyclonal anti-mGluR5 (1:200, EMD Millipore), goat monoclonal anti-Iba-1 (1:200; Abcam), mouse monoclonal anti-α-syn (1:100, BD Biosciences), rat monoclonal anti-lysosome-associated membrane protein (LAMP)-1 (1:50, Santa Cruz Biotechnology), Cell-Signaling:Article Title: A novel cholinergic projection from the lateral parabrachial nucleus and its role in methamphetamine-primed conditioned place preference Article Snippet: .. The following primary antibodies were used: goat polyclonal anti-ChAT (1:200, RRID: AB_2079751, Millipore, USA), Blocking Assay:Article Title: Astrocytic mGluR5 signaling tunes emotional and cognitive processing in the adult brain Article Snippet: Free-floating tissue was first washed in PBS and then permeabilized with a 0.3% Triton X-100 (Sigma Aldrich, USA) in PBS (0.3% PBS-T) solution for 10 min. To reduce nonspecific binding, the tissue was incubated with 10% normal goat serum (NGS, Abcam) in 0.3% PBS-T blocking solution for 1 h at room temperature (RT). .. This blocking step was followed by overnight incubation at 4 °C with the primary antibodies rabbit anti-GFAP (1:200, Abcam, UK), rabbit anti-mGluR5 (1:200), Incubation:Article Title: Astrocytic mGluR5 signaling tunes emotional and cognitive processing in the adult brain Article Snippet: Free-floating tissue was first washed in PBS and then permeabilized with a 0.3% Triton X-100 (Sigma Aldrich, USA) in PBS (0.3% PBS-T) solution for 10 min. To reduce nonspecific binding, the tissue was incubated with 10% normal goat serum (NGS, Abcam) in 0.3% PBS-T blocking solution for 1 h at room temperature (RT). .. This blocking step was followed by overnight incubation at 4 °C with the primary antibodies rabbit anti-GFAP (1:200, Abcam, UK), rabbit anti-mGluR5 (1:200), Article Title: Adenosine deaminase acting on RNA 2 provides neuroprotection by activating Kv1.1 channels in a rat epilepsy model Article Snippet: .. The sections were blocked with 3% (v/v) bovine serum albumin (BSA) and incubated with 100 mL diluted primary antibody of Article Title: Genetically modified mouse expressing human APOE4 and mouse Trem2 p.R47H and methods of use thereof Article Snippet: Cryosections were rinsed with PBT (1×PBS with 1% Triton-100) for 5 minutes (mins) then incubated with 500 μL of Liberate Antibody Binding Solution (L.A.B.-Polysciences Inc.) solution for 20 minutes at room temperature (RT) for antigen retrieval. .. Slides were then incubated overnight at 4° C. in the following primary antibodies: rabbit polyclonal anti-GFAP (1:200, Dako); rabbit polyclonal anti-IBA1 (1:250, Wako); Next-Generation Sequencing:Article Title: Astrocytic mGluR5 signaling tunes emotional and cognitive processing in the adult brain Article Snippet: Free-floating tissue was first washed in PBS and then permeabilized with a 0.3% Triton X-100 (Sigma Aldrich, USA) in PBS (0.3% PBS-T) solution for 10 min. To reduce nonspecific binding, the tissue was incubated with 10% normal goat serum (NGS, Abcam) in 0.3% PBS-T blocking solution for 1 h at room temperature (RT). .. This blocking step was followed by overnight incubation at 4 °C with the primary antibodies rabbit anti-GFAP (1:200, Abcam, UK), rabbit anti-mGluR5 (1:200), Immunofluorescence:Article Title: Demonstration of therapeutic effect of plasma-synthesized polypyrrole/iodine biopolymer in rhesus monkey with complete spinal cord section. Article Snippet: .. Immunofluorescence assay was done in 8 mm sagittal sections of spinal cord by using specific primary antibodies for neurons: |


